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KMID : 1059519860300050369
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Journal of the Korean Chemical Society
1986 Volume.30 No. 5 p.369 ~ p.375
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A Study on Capillary Gas-Liquid Chromatographic Determination of Diosgenin in Costus Speciosus
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Kim Taek-Jae
Cha Kee-Surk
Kim Young-Sang
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Abstract
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Diosgenin in an Indonesian Costus speciosus was determined by capillary gas-liquid chromatography (GLC). The experimental conditions for the hydrolysis, extraction and acetylation of the diosgenin, and the determination by GLC were investigated. 0.20g of dried sample powder was refluxed in the solution of 3N HCI and xylene at 95∼100℃ for 4 hours and the xylene layer was separated. The residue evaporated the xylene was refluxed in 20 : 80 acetic anhydride-pyridine for 30 minutes and the diosgenin acetate was extracted with diethyl ether. Dehydrated with anhydrous Na2SO4 and evaporated the ether, the residue was dissolved in 5.00ml of n-hexane and injected into GLC. Capillary column of SE-30 25m × 0. 33mm was installed in GLC and the column temperature was increased from 180° to 270℃ at rate of 10℃/min. The flow rate of carrier gas N2 was 2ml/min and FID was used to detect. The analytical result of the diosgenin was 0.281% and relative standard deviation of 5 measures was 1.8%.
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